广东畜牧兽医科技 ›› 2023, Vol. 48 ›› Issue (4): 13-19.DOI: 10.19978/j.cnki.xmsy.2023.04.03

• • 上一篇    下一篇

不同温度对柔嫩艾美耳球虫卵囊活力的影响

林栩慧1# ,朱易斌1# ,郭素茵3 ,戚南山1 ,郭钊文3 ,李娟1 ,严专强2 , 尹丽娟2 ,李汝洪3 ,刘佐正4 ,吕敏娜1 ,廖申权1,2* ,孙铭飞1*   

  1. (1.广东省农业科学院动物卫生研究所/广东省畜禽疫病防治研究重点实验室/农业农村部禽流 感等家禽重大疾病防控重点实验室,广东 广州,510640; 2. 岭南现代农业科学与技术广东省实验室云浮分中心,广东 云浮 527400; 3 .清远市清城区动物防疫中心,广东 清远 511500; 4. 河源市温氏畜禽有限公司,广东 河源 517000)
  • 出版日期:2023-08-17 发布日期:2023-08-18
  • 通讯作者: 廖申权,孙铭飞
  • 基金资助:
    “十四五”广东省农业科技创新十大主攻方向“揭榜挂帅”项目(NO. 2022SDZG02);广东省农业科学院协同创新中心项目 (XTXM202202);科技创新战略专项资金(高水平农科院建设)(202110TD,202122TD,R2020PY ? JC001,R2019YJ ?YB3010,R2020PY ? JG013,R2020QD?048,R2021PY?QY007,R2023PY?JG018);广东省现代农业产业技术体系创新团队建设专项(2022KJ119)

Effects of Different Temperatures on Viability and Sporulation of Oocysts from Eimeria Tenella

LIN Xuhui1# ,ZHU Yibin1# ,GUO Suyin3 ,QI Nanshan1 ,GUO Zhaowen3 ,LI Juan 1 ,YAN Zhuanqiang 2 ,YIN Lijuan2 ,LI Ruhong3 ,LIU Zuozheng4 ,LV Minna1 ,LIAO Shenquan1,2* ,SUN Mingfei1*   

  1. (1.Key Laboratory of Livestock Disease Prevention of Guangdong Province,Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province,Key Laboratory of Avian Influenza and Other Major Poultry Diseases Prevention and Control,Ministry of Agriculture and Rural Affairs,Institute of Animal Health,Guangdong Academy of Agricultural Sciences,Guangzhou Guangdong 510640; 2.Yunfu Branch Guangdong Laboratory for Lingnan Modern Agriculture,Yunfu Guangdong 527400;3.Qingcheng district animal epidemic prevention center,Qingyuan Guangdong 511500; 4.Wen's Livestock and Poultry Co.,Ltd.,Heyuan Guangdong 517000)
  • Online:2023-08-17 Published:2023-08-18

摘要: 环境温度是影响鸡球虫卵囊孢子化和活力的重要因素,该研究通过监测鸡球虫卵 囊孢子化率、鸡感染球虫后的肠道病变以及卵囊排出情况,探讨不同温度对柔嫩艾美耳球虫未 孢子化卵囊的孢子化作用以及对孢子化卵囊的活性影响。结果表明:未孢子化卵囊分别经过 -80 ℃处理 30 min,-40 ℃、-20 ℃、0 ℃、4 ℃、10 ℃、28 ℃、40 ℃、50 ℃处理 30 min 或 1 h 后,孢子化活性均无显著变化;未孢子化卵囊分别经过-80 ℃处理1 h以上、-40 ℃或-20 ℃处 理48 h以上、60 ℃处理30 min以上后孢子化活性被抑制;孢子化卵囊经液氮处理2 min、-80 ℃ 或高于50 ℃条件处理超过1 h,卵囊活力基本为0,在-20 ℃处理1—12 h或50 ℃处理30 min 卵囊仍表现有一定活力。该研究为鸡球虫卵囊保存条件提供数据支持,对防治鸡球虫病具有 一定的研究意义。

关键词: 柔嫩艾美耳球虫, 不同温度, 卵囊活力

Abstract: Temperature is an important factor affecting the sporulation and viability of avian coccidia oocysts. In this study,the effects of different temperatures on the sporulation of Eimeria tenella unsporulated oocysts and the viability of sporulated oocysts were investigated by monitoring the sporulation rate of avian coccidia oocysts,intestinal lesions and oocyst production. The results showed that there was no significant change in the oocyst sporulation activity compared to be treated at -80 ℃ for 30 min,and at -40 ℃,-20 ℃,0 ℃,4 ℃,10 ℃,28 ℃,40 ℃,50 ℃ for 30 min or 1 h respectively,but the oocyst sporulation activity was inhibited after administrated at -80 ℃ for more than 1 h,- 40 ℃ or - 20 ℃ for more than 48 h,and 60 ℃ for more than 30 min. The sporulated oocysts were killed by exposure to liquid nitrogen for 2 min,or at -80 ℃ or higher than 50 ℃ for more than 1 h,whereas sporulated oocysts remain viable at -20 ℃ for 1 to 12 h or at 50 ℃ for 30 min. This study provides data support for the storage conditions of avian coccidia oocysts,and has certain research significance for the prevention and treatment of avian coccidiosis.

Key words: Eimeria tenella, Temperature, Vitality, Sporulation

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