关键词: 猪伪狂犬病活疫苗； 生产用毒种； 安全性检验； 免疫原性检验； 生物学特 性

Abstract: To study the biological characteristics of different generations of porcine pseudorabies live vaccinetemperature of piglets in the immune group were normal after post ?infection with PRV GD1 strain；while the body temperature of piglets in the control group was increased after post ?infection with PRV GD1 strain，and all of them became ill and died. These results indicated that the different generations of porcine pseudorabies live vaccine（Bartha? K61 strain，cell line origin）production virus seeds were safe and maintained good immunogenicity to piglets. （Bartha ? K61 strain，cell line origin）production virus seeds，the porcine pseudorabies live vaccine（Bartha ? K61 strain，cell line origin）basic virus seed F7 was continuously passaged to F13，and six generations of PRV F8—PRV F13 were inoculated with piglets for safety test，and three generations of PRV F8、PRV F10 and PRV F13 were inoculated with piglets for immunogenicity test. The results of the safety test showed that the spirit，appetite，and body temperature of piglets were normal during the observation period after each generation of production virus seeds were inoculated on the neck muscles；the results of the immunogenicity test showed that the spirit，appetite，and body

Key words: Porcine pseudorabies live caccine； Production virus seeds； Safety test； Immunogenicity test； Biological characteristics