广东畜牧兽医科技 ›› 2023, Vol. 48 ›› Issue (4): 30-34.DOI: 10.19978/j.cnki.xmsy.2023.04.06

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贝莱斯芽孢杆菌的鉴定和抑菌作用研究

周淑贞1 ,辜质纯1 ,张瑞雪2 ,廖洁丹3 ,谢志恒1 ,张悻2 ,顾万军3 ,黄良宗3*   

  1. (1. 佛山市南海东方澳龙制药有限公司,广东 佛山 528234; 2. 佛山市南海区维德生物技术有限公司,广东 佛山 528234; 3. 佛山科学技术学院 生命科学与工程学院,广东 佛山 528231)
  • 出版日期:2023-08-17 发布日期:2023-08-18
  • 通讯作者: 黄良宗
  • 基金资助:
    广东省现代草牧业(羊)产业技术体系项目(2019KJ127)

Identification and Antimicrobial Test of Bacillus Velezensis

ZHOU Shuzhen1 ,GU Zhichun1 ,ZHANG Ruixue2 ,LIAO Jiedan3 ,XIE Zhiheng 1 ,ZHANG Xing2 , GU Wanjun3 ,HUANG Liangzong3**   

  1. (1. Eastern Along Pharmaceutical Co.,Ltd.,Nanhai,Foshan Guangdong 528234; 2. Foshan Nanhai District Weide Bio?Technology Co.,Ltd,Guangdong 528234; 3. Foshan University School of Life Science and Engineering,Guangdong 528231)
  • Online:2023-08-17 Published:2023-08-18

摘要: 该研究对6株贝莱斯芽孢杆菌分离株进行测序鉴定、药敏试验与抑菌试验。结果表 明:所分离的6株菌16S rDNA序列与贝莱斯芽孢杆菌处于同一遗传进化树分支,对选定的7种 抗菌药物均敏感。应用指示菌进行抑菌试验,6株菌及其代谢产物对金黄色葡萄球菌和大肠杆 菌均有抑菌作用,抑菌作用从强到弱依次是:贝莱斯芽孢杆菌菌液、上清液和菌液滤液。

关键词: 贝莱斯芽孢杆菌; 鉴定; 抑菌试验

Abstract: In this study,sequencing identification,drug sensitivity testing,and antibacterial assays were conducted on six Bacillus velezensis strains isolated from diverse sources. The results indicated that the 16S rDNA sequences of these six isolates were positioned within the same genetic evolutionary branch of Bacillus velezensis,and sensitivity to seven selected antimicrobial drugs was exhibited. Bacteriostatic effects against both Staphylococcus aureus and Escherichia coli were demonstrated for the six strains and their metabolites. The bacteriostatic intensity of the samples followed the order of Bacillus velezensis liquid, supernatant,and filtrate.

Key words: Bacillus velezensis; Identification; Antibacterial test

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